Signalling

Part:BBa_K1909011

Designed by: Daniel Wedemeyer   Group: iGEM16_Hamburg   (2016-10-14)


Medium Anderson / Taz

Ba_K1909011 is a construct of medium Anderson promoter BBa_K608006, a strong ribosome binding site and the receptor tar-envZ, part BBa_C0082, which is a combination of the chemotaxis receptor Tar capable of sensing aspartate, and the cytosolic domain of osmosensor kinase EnvZ.

Functional Parameters

This combination has a higher expression as BBa_K1909012 and a lower expression as BBa_K1909010. Testing the combination showed that the cells grew slowly (for miniprep culture more than 24 hours). We assume that this combination (medium promoter and strong RBS) induces a high expression of the transmembrane receptor. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 173


[edit]
Categories
//cds/membrane/receptor
//cds/receptor
//chassis/prokaryote/ecoli
Parameters
chassisE. coli JW 3367-3